Cytokine Induced Memory-like NK Cell Adoptive Therapy for Relapsed AML After Allogeneic Hematopoietic Cell Transplant

Recipient Inclusion Criteria:

Relapsed AML after HLA-matched related or unrelated allogeneic hematopoietic cell transplant
For pilot pediatric/young adult patient cohort ≥1 and <18 years of age
For phase 2 adult patient cohort ≥18 years of age
Available original donor (same donor as used for the initial stem cell transplant) that is willing and eligible for non-mobilized collection
Patients with known central nervous system (CNS) involvement with AML are eligible provided that they have been treated and cerebrospinal fluid (CSF) is clear for at least 2 weeks prior to enrollment into the study. CNS therapy (chemotherapy or radiation) should continue as medically indicated during the study treatment.
Karnofsky performance status > 60 %

Adequate organ function as defined below:

Total bilirubin < 2 mg/dL
AST(SGOT)/ALT(SGPT) < 3.0 x IULN
Creatinine within normal institutional limits OR creatinine clearance > 60 mL/min/1.73 m2 by Cockcroft-Gault Formula
Oxygen saturation ≥90% on room air
Not currently requiring systemic corticosteroid therapy (10 mg or less of prednisone or equivalent doses of other systemic steroids are allowed) or any other immune suppressive medications
Women of childbearing potential must have a negative pregnancy test within 28 days prior to study registration. Female and male patients (along with their female partners) must agree to use two forms of acceptable contraception, including one barrier method, during participation in the study including throughout the initial evaluation period (100 days after CIML NK cell infusion).
Ability to understand and willingness to sign an IRB approved written informed consent document (or that of legally authorized representative, if applicable).

Recipient Exclusion Criteria:

Acute or chronic GvHD with ongoing active systemic treatment.
Circulating blast count >10,000/uL by morphology or flow cytometry (cyto-reductive therapies, including salvage chemotherapy, is encouraged prior to study enrollment)
Uncontrolled bacterial or viral infections, or known HIV, Hepatitis B, or Hepatitis C infection.
Uncontrolled angina, severe uncontrolled ventricular arrhythmias, or EKG suggestive of acute ischemia or active conduction system abnormalities.
New or progressive pulmonary infiltrates concerning for new or uncontrolled infectious process.
Known hypersensitivity to one or more of the study agents
Received any investigational drugs within the 14 days prior to CIML NK cell infusion date
Pregnant and/or breastfeeding

Donor Inclusion Criteria:

At least 18 years of age
Same donor as used for the allo-HCT
In general good health, and medically able to tolerate leukapheresis
Ability to understand and willingness to sign an IRB approved written informed consent document

Donor Exclusion Criteria:

Active hepatitis, positive for HTLV, or HIV on donor viral screen
Pregnant