Myeloid-Derived Suppressor Cells and Checkpoint Immune Regulators’ Expression in Allogeneic SCT Using FluBuATG

Inclusion Criteria:

Age less than or equal to 75 years
The patient must be approved for transplant by the treating transplant physician. This includes completion of their pretransplant workup, as directed by standard Dartmouth-Hitchcock Medical Center (DHMC) Standard Operating Procedures (SOPs). DHMC SOP for Pretransplant Evaluation of allogeneic recipient.

The patient must have a disease, listed below, with treatment responsiveness that the treating transplant physician believes will benefit from an allogeneic stem cell transplant. The diseases include:

Acute leukemia AML (Acute Myeloid Leukemia), ALL (Acute Lymphoid Leukemia)
Chronic leukemia CML (Chronic Myeloid Leukemia), CLL (Chronic Lymphoid Leukemia)
Myelodysplasia
Myelofibrosis
Lymphoma NHL (Non-Hodgkin's Lymphoma) and Hodgkin's disease
Plasma cell disorder, including myeloma, Waldenstrom's Macroglobulinemia

Donor availability- the patient must have an identified donor

Sibling Availability of a 6 out of 6 identical donor
Unrelated donor: Availability of a 6 out of 6 unrelated donor
No human immunodeficiency virus (HIV) infection or active hepatitis B or C
Easter Cooperative Oncology Group (ECOG) performance status 0, 1, or 2
Diffusing capacity of the lungs for carbon monoxide DLCO more than or equal to 40 percent predicted
Left ventricular ejection fraction more than or equal to 35 percent
Serum bilirubin less than 2x upper limit of normal transaminases less than 3x normal at the time of transplant
No active or uncontrollable infection
In female, a negative pregnancy test if experiencing menstrual periods
No major organ dysfunction precluding transplantation
No evidence of an active malignancy that would limit the patient's survival to less than 2 years. If there is any question, the principal investigator can make a decision.

Exclusion Criteria:

Psychiatric disorder or a mental deficiency of the patient that is sufficiently severe to make compliance with the treatment unlikely, and making informed consent impossible.
Major anticipated illness or organ failure incompatible with survival from bone marrow transplant.
History of refractory systemic infection

Donor eligibility

Human leukocyte antigen (HLA) 6 out of 6 matched related or unrelated donor.
The donor must be healthy and must be willing to serve as a donor, based on standard guidelines
The donor must have no significant comorbidities that would put the donor at marked increased risk
There is no age restriction for the donor

Informed consent must be signed by donor, if sibling donor, or by third party if unrelated donor.

Donor Exclusion Criteria

The National Marrow Donor Program (NMDP) guidelines for exclusion criteria will be used. In addition, the following donors are NOT eligible:
Syngeneic donor
Pregnant or lactating donor
Human immunodeficiency virus (HIV) or active HepB or C in the donor
Donor unfit to receive Granulocyte-colony stimulating factor (GCSF) and undergo apheresis
A donor with a psychiatric disorder or mental deficiency that makes compliance with the procedure unlikely and informed consent impossible