This pilot research trial utilizes the collection of blood in studying metabolites in patients with prostate cancer. Metabolites are the small molecule products of cellular metabolism that are produced naturally in all living cells. Collecting blood in order to study metabolites may help doctors monitor and treat prostate cancer more effectively.
I. Develop blood sample collection and preparation procedures for reliable, meaningful metabolomic profiling of peripheral blood mononuclear cells (PBMCs) by liquid chromatography/quadruple-time of flight/mass spectrometry (LC/Q-TOF/MS) that can be implemented in a clinical setting.
II. Optimize data analysis methods and software usage to create a metabolic profile for patients at the time of blood collection.
III. Compare metabolite profiles of isolated PBMCs versus (vs.) other blood fractions (plasma, red blood cells [RBCs], and whole blood).
IV. Identify elements of the PBMC metabolite profile that may correlate to patient disease state.
OUTLINE:
Patients undergo collection of blood for metabolic profiling via LC/Q-TOF/MS.
All subjects will have histologically confirmed prostate cancer Provide written consent/authorization to participate in this study Have no signs or symptoms of active infection Exhibit a willingness to comply with the experimental procedures as outlined in this protocol
